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maxima rt buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher maxima rt buffer
    Maxima Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxima+rt+buffer/maxima+rt+buffer/pmc10104825__41467_2023_37749_MOESM1_ESM-72-16-29
    Average 90 stars, based on 1 article reviews
    maxima rt buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Participation of preovulatory follicles in the activation of primordial follicles in mouse ovaries
    Article Snippet: .. The template switch reaction mix consisted of 93.5 μL of 30% PEG6000, 44 μL of Maxima RT buffer (Thermo Fisher, EP0753), 44 μL of Ficoll PM-400 solution (Sigma-Aldrich, F5415), 11 μL of dNTPs (Thermo Fisher, N0447S), 5.5 μL of RNase Inhibitor (Enzymatics, Y9420L), 11 μL of Maxima H Minus Reverse Transcriptase (Thermo Fisher, EP0753), and 11 μL of template switch primer. ..

    Article Title: Single cell transcriptional profiling of benign prostatic hyperplasia reveals a progenitor-like luminal epithelial cell state within an inflammatory microenvironment
    Article Snippet: .. For each array, beads were washed with Maxima RT buffer (ThermoFisher, Cat: EP0753) and resuspended in reverse transcription mastermix with Maxima RT buffer, PEG8000, Template Switch Oligo, dNTPs (NEB, Cat: N0447L), RNase inhibitor (Life Technologies, Cat: AM2696) and Maxima H Minus Reverse Transcriptase (ThermoFisher, Cat: EP0753) in water. ..

    other:

    Article Title: Scalable co-sequencing of RNA and DNA from individual nuclei
    Article Snippet: Of this, 23 μl was mixed with reverse transcription master mix (1.25 μl of 10 μM scc primers, 10 μl Maxima RT buffer (Thermo Fisher, EP0753), 5 μl dNTPs (New England Biolabs, N0447L), 2.5 μl SUPERase·In, 1.25 μl SMART TSO, 2.5 μl Maxima H Minus Reverse Transcriptase (Thermo Fisher, EP0753) and 0.5 μl 10% Tween 20 (Millipore Sigma, P9416)).

    Article Title: Mesenchymal GDNF promotes intestinal enterochromaffin cell differentiation
    Article Snippet: Bead mixture was combined with reverse transcriptase (RT) mix (1 × Maxima RT buffer, Maxima H- RTase (Thermo Fisher, EP0743), RiboLock RNase inhibitor (Thermo Fisher, EO0382), 10 mM dNTPs (Thermo Fisher, R0193) and 50 μM Template Switch Oligo (5M Betaine Solution 15825238)).

    Article Title: Histone variant H2BE controls activity-dependent gene expression and homeostatic scaling
    Article Snippet: For up to 120,000 beads, 200 μL of reverse transcription (RT) mix (1x Maxima RT buffer (ThermoFisher), 4% Ficoll PM-400, 1 mM dNTPs (Clontech), 1 U/mL RNase inhibitor, 2.5 mM Template Switch Oligo (TSO: AAGCAGTGGTATCAACGCAGAGTGAATrGrGrG), and 10 U/ mL Maxima H Minus Reverse Transcriptase (ThermoFisher)) were added.

    Lysis:

    Article Title: Mesenchymal GDNF promotes intestinal enterochromaffin cell differentiation
    Article Snippet: .. Indexing Oligobeads (15μL) were added to the cell lysis mix and after 5 min incubation the beads were collected with a magnet and washed 2 times with 6× Saline-sodium citrate (SSC) (Invitrogen, 15557044) and once with 1× Maxima RT Buffer (Thermo Fisher, EP0743). .. Bead mixture was combined with reverse transcriptase (RT) mix (1 × Maxima RT buffer, Maxima H- RTase (Thermo Fisher, EP0743), RiboLock RNase inhibitor (Thermo Fisher, EO0382), 10 mM dNTPs (Thermo Fisher, R0193) and 50 μM Template Switch Oligo (5M Betaine Solution 15825238)).

    Incubation:

    Article Title: Mesenchymal GDNF promotes intestinal enterochromaffin cell differentiation
    Article Snippet: .. Indexing Oligobeads (15μL) were added to the cell lysis mix and after 5 min incubation the beads were collected with a magnet and washed 2 times with 6× Saline-sodium citrate (SSC) (Invitrogen, 15557044) and once with 1× Maxima RT Buffer (Thermo Fisher, EP0743). .. Bead mixture was combined with reverse transcriptase (RT) mix (1 × Maxima RT buffer, Maxima H- RTase (Thermo Fisher, EP0743), RiboLock RNase inhibitor (Thermo Fisher, EO0382), 10 mM dNTPs (Thermo Fisher, R0193) and 50 μM Template Switch Oligo (5M Betaine Solution 15825238)).

    Article Title: Repurposing host-guest chemistry to sequester virulence and eradicate biofilms in multidrug resistant Pseudomonas aeruginosa and Acinetobacter baumannii
    Article Snippet: Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides (Integrated DNA Technologies). .. After 5 minutes of incubation at ambient temperature, RNA was combined with RT mix containing 1× Maxima RT buffer, 1 mM dNTPs, 10 U μL-1 Maxima H- RTase (all Thermo Fisher Scientific), 1 U μL-1 RNase inhibitor (Lucigen) and 2.5 μM Template Switch Oligo (Integrated DNA Technologies). .. Samples were incubated in a T100 thermal cycler (BioRad) for 30 minutes at 22°C, then 90 minutes at 52°C.

    Saline:

    Article Title: Mesenchymal GDNF promotes intestinal enterochromaffin cell differentiation
    Article Snippet: .. Indexing Oligobeads (15μL) were added to the cell lysis mix and after 5 min incubation the beads were collected with a magnet and washed 2 times with 6× Saline-sodium citrate (SSC) (Invitrogen, 15557044) and once with 1× Maxima RT Buffer (Thermo Fisher, EP0743). .. Bead mixture was combined with reverse transcriptase (RT) mix (1 × Maxima RT buffer, Maxima H- RTase (Thermo Fisher, EP0743), RiboLock RNase inhibitor (Thermo Fisher, EO0382), 10 mM dNTPs (Thermo Fisher, R0193) and 50 μM Template Switch Oligo (5M Betaine Solution 15825238)).



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